Fun in the Lab
At the moment I have a technicians job, working in a lab. I have two weeks left, and must Finnish the tasks that I have been charged.
- Create a wild type clone of a region of exonic human sequence.
- Induce SNP into three wild type clone via the quick change protocol.
- Write a workflow that produced a list of ranked candidate genes, for a human disease using tools such as blast , open reading frame finder tools, and motif searchers.
Currently I am having trouble carrying out my ligation reaction into a PGemT easy vector system and then transforming it. Transformation and cells are fine as demonstrated by controls. Ligation is the issue. Diagnostic restriction digests are currently not working, I think this is due to not having enough DNA to digest and then visualise on the gel.
Quick change has been performed on 2 of the three wt clones, and clones have been isolated. The gene now just needs to be sent for sequenceing.
Workflow is on hold.